Application
Immunohistochemistry of Spred1 in human brain tissue with Spred1 antibody at 2.5 μg/mL.Application
Immunofluorescence of Spred1 in Human Brain tissue with Spred1 antibody at 20 μg/mL.Application
Western blot analysis of Spred1 in human brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Spred1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Spred1 Antibody: NFLS, hSpred1, spred-1, Sprouty-related, EVH1 domain-containing protein 1, Spred-1 |
| Product description | Spred1 Antibody: The Ras-MAP kinase pathway is essential for the differentiation of neuronal cells and myocytes; it is inhibited by Spred1, a member of the Sprouty family of proteins. Spred1 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. Defects in this gene are a cause of neurofibromatosis type 1-like syndrome (NFLS).1) Wakioka T, Sasaki A, Kato R, et al. Spred is a Sprouty-related suppressor of Ras signalling. Nature 2001; 412:647-51. |
| Immunogen | Spred1 antibody was raised against a 14 amino acid synthetic peptide near the center of the human Spred1. The immunogen is located within amino acids 250 - 300 of Spred1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPRED1 |
|---|---|
| Alternative Names | Spred1 Antibody: NFLS hSpred1 spred-1 Sprouty-related EVH1 domain-containing protein 1 Spred-1 |
| Molecular Weight(MW) | Predicted: 49 kDa Observed: 498 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This Spred1 antibody is predicted to have no cross-reactivity to Spred2 or Spred3. |
Application
Immunohistochemistry of Spred1 in human brain tissue with Spred1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of Spred1 in Human Brain tissue with Spred1 antibody at 20 μg/mL.
Application
Western blot analysis of Spred1 in human brain tissue lysate with Spred1 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | Spred1 antibody can be used for detection of Spred1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Spred1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Spred1 Antibody is supplied in PBS containing 0.02% sodium azide. |
