Application
Western blot analysis of Spred2 in human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | Spred2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Spred2 Antibody: Spred-2, Sprouty-related, EVH1 domain-containing protein 2, Spred-2 |
| Product description | Spred2 Antibody: Spred2 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1, Spred2 acts by suppressing the phosphorylation and activation of Raf. The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Further studies have shown that expression levels of Spred1 and Spred2 proteins are inversely correlated with the incidence of tumor invasion and metastasis in human hepatocellular carcinoma (HHC), suggesting that these proteins could be useful as prognostic factors and therapeutic targets in HCC. At least two isoforms of Spred2 are known to exist.1) Wakioka T, Sasaki A, Kato R, et al. Spred is a Sprouty-related suppressor of Ras signalling. Nature2001; 412:647-51. |
| Immunogen | Spred2 antibody was raised against a 13 amino acid synthetic peptide near the center of the human Spred2. The immunogen is located within amino acids 140 - 190 of Spred2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| Spred2 antibody can be used for detection of Spred2 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPRED2 |
|---|---|
| Alternative Names | Spred2 Antibody: Spred-2 Sprouty-related EVH1 domain-containing protein 2 Spred-2 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse: (85%), Rat: (85%) |
| Tissue Specificity | This Spred2 antibody is predicted to have no cross-reactivity to Spred1 or Spred3. |
Application
Western blot analysis of Spred2 in human small intestine tissue lysate with Spred2 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1308 - Human Small Intestine Tissue Lysate |
|---|---|
| Application Notes | Spred2 antibody can be used for detection of Spred2 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Spred2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Spred2 Antibody is supplied in PBS containing 0.02% sodium azide. |
