Application
Immunohistochemistry of Spred3 in human brain tissue with Spred3 antibody at 2.5 μg/mL.Application
Immunofluorescence of spred3 in human brain tissue with spred3 antibody at 20 μg/mL.Application
Western blot analysis of Spred3 in mouse brain tissue lysate with Spred3 antibody at (A) 2 and (B) 4 μg/mL.| Product Name | Spred3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Spred3 Antibody: Eve-3, spred-3, EVE-3, Sprouty-related, EVH1 domain-containing protein 3, Spred-3 |
| Product description | Spred3 Antibody: Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD). The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist.1) Kato R, Nonami A, Taketomi T, et al. Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem. Biophys. Res. Commun.2003; 302:767-72. |
| Immunogen | Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3. The immunogen is located within amino acids 140 - 190 of Spred3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPRED3 |
|---|---|
| Alternative Names | Spred3 Antibody: Eve-3 spred-3 EVE-3 Sprouty-related EVH1 domain-containing protein 3 Spred-3 |
| Molecular Weight(MW) | Predicted: 45 kDa Observed: 45 kDa |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2. |
| Entrez Gene | 399473 |
|---|---|
| Protein Accession | NP_001035987 |
Application
Immunohistochemistry of Spred3 in human brain tissue with Spred3 antibody at 2.5 μg/mL.
Application
Immunofluorescence of spred3 in human brain tissue with spred3 antibody at 20 μg/mL.
Application
Western blot analysis of Spred3 in mouse brain tissue lysate with Spred3 antibody at (A) 2 and (B) 4 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Spred3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Spred3 Antibody is supplied in PBS containing 0.02% sodium azide. |
