SPRY3 contains 1 B30.2/SPRY domain. Sprouty/Caveolin-1 interaction modulates signaling in a growth factor- and Sprouty isoform-specific manner. Spry 1, 2 and 3 expression was observed in placental tissue from all three trimestersl. The exact function of SPRY3 remains unknown.1) Cabrita, M.A., (2006) J. Biol. Chem. 281 (39), 29201-22912.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPRY3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SPRY3 antibody can be used for detection of SPRY3 by ELISA at 1:62500. SPRY3 antibody can be used for detection of SPRY3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Application Notes
SPRY3 antibody can be used for detection of SPRY3 by ELISA at 1:62500. SPRY3 antibody can be used for detection of SPRY3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPRY3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.