Application
Immunofluorescence of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 20 μg/mL.Application
Immunohistochemistry of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 2.5 μg/mL.Application
Western blot analysis of SPRYD2 in mouse heart tissue lysate with SPRYD2 antibody at 1 μg/mL.| Product Name | SPRYD2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPRYD2 Antibody: SPRYD2, TRIM76, C5orf10, DTNBP2, SPRYD2, Cardiomyopathy-associated protein 5, Dystrobrevin-binding protein 2 |
| Product description | SPRYD2 Antibody: SPRYD2, also known as Myospryn, was originally identified as the muscle-specific partner of dysbindin and as a Mef-2 target gene. It is a large scaffolding protein localized to the Z-disc/costamere region of striated muscle. SPRYD2 includes a noncanonical tripartite motif (TRIM-like) that lacks the RING domain but consists of a B-box coiled coil (BBC), fibronectin 3 (FN3) repeats, and SPRY domains. SPRYD2 interacts with desmin and calcineurin, and it has been suggested to play a role in the biogenesis of lysosome and negatively regulates slow-fiber-type transformation and skeletal muscle regeneration. SPRYD2 is dysregulated in Duchenne muscular dystrophy.1) Benson MA, Tinsley CL, Blake DJ. Myospryn is a novel binding partner for dysbindin in muscle. J. Biol. Chem. 2004; 279:10450-8. |
| Immunogen | SPRYD2 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD2. The immunogen is located within amino acids 3810 - 3860 of SPRYD2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SPRYD2 antibody can be used for detection of SPRYD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CMYA5 |
|---|---|
| Alternative Names | SPRYD2 Antibody: SPRYD2 TRIM76 C5orf10 DTNBP2 SPRYD2 Cardiomyopathy-associated protein 5 Dystrobrevin-binding protein 2 |
| Tissue Specificity | SPRYD2 antibody is predicted to not cross-react with other SPRYD protein family members. At least four isoforms of SPRYD2 are known to exist. |
Application
Immunofluorescence of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 20 μg/mL.
Application
Immunohistochemistry of SPRYD2 in mouse brain tissue with SPRYD2 antibody at 2.5 μg/mL.
Application
Western blot analysis of SPRYD2 in mouse heart tissue lysate with SPRYD2 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | SPRYD2 antibody can be used for detection of SPRYD2 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPRYD2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPRYD2 Antibody is supplied in PBS containing 0.02% sodium azide. |
