Application
Immunohistochemistry of SPRYD4 in human kidney tissue with SPRYD4 antibody at 2.5 μg/mL.Application
Immunofluorescence of SPRYD4 in human kidney tissue with SPRYD4 antibody at 20 μg/mL.Application
Western blot analysis of SPRYD4 in fetal human liver tissue lysate with SPRYD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Product Name | SPRYD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPRYD4 Antibody: SPRY domain-containing protein 4 |
| Product description | SPRYD4 Antibody: The SPRY domain-containing protein 4 (SPRYD4) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD4 is ubiquitously expressed and is most abundant in kidney, brain, bladder, thymus and stomach. Little is known of the function of the SPRYD4 protein.1) Tae H, Casarotto MG, and Dulhunty AF. Ubiquitous SPRY domains and their role in the skeletal type ryanodine receptor. Eur. Biophys. J. 2009; 39:51-9. |
| Immunogen | SPRYD4 antibody was raised against an 18 amino acid synthetic peptide near the center of human SPRYD4. The immunogen is located within amino acids 70 - 120 of SPRYD4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SPRYD4 antibody can be used for detection of SPRYD4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPRYD4 |
|---|---|
| Alternative Names | SPRYD4 Antibody: SPRY domain-containing protein 4 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
| Tissue Specificity | SPRYD4 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD4 are known to exist; this antibody will detect both isoforms. |
Application
Immunohistochemistry of SPRYD4 in human kidney tissue with SPRYD4 antibody at 2.5 μg/mL.
Application
Immunofluorescence of SPRYD4 in human kidney tissue with SPRYD4 antibody at 20 μg/mL.
Application
Western blot analysis of SPRYD4 in fetal human liver tissue lysate with SPRYD4 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | SPRYD4 antibody can be used for detection of SPRYD4 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPRYD4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPRYD4 Antibody is supplied in PBS containing 0.02% sodium azide. |
