Application
Immunofluorescence of SPRYD5 in mouse brain tissue with SPRYD5 antibody at 20 μg/mL.Application
Immunohistochemistry of SPRYD5 in mouse brain tissue with SPRYD5 antibody at 5 μg/mL.Application
Western blot analysis of SPRYD5 in rat brain tissue lysate with SPRYD5 antibody at (A) 0.25 and (B) 0.5 μg/mL .| Product Name | SPRYD5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPRYD5 Antibody: SPRYD5, TRIM51A, SPRYD5, Tripartite motif-containing protein 51, SPRY domain-containing protein 5 |
| Product description | SPRYD5 Antibody: The SPRY domain-containing protein 5 (SPRYD5) is a member of a family of proteins whose sole common characteristic is the presence of a SPRY domain. SPRY domains are structural domains that were first described in the fungal Dictyostelium discoideum tyrosine kinase spore lysis A. In most systems SPRY domains provide binding sites for regulatory proteins or intramolecular binding sites that maintain the structural integrity of a protein. SPRYD5 belongs to the TRIM/RBCC family and contains one B box-type zinc finger, one B30.2/SPRY domain and one RING-type zinc finger. Little is known of the function of the SPRYD5 protein.1) Tae H, Casarotto MG, and Dulhunty AF. Ubiquitous SPRY domains and their role in the skeletal type ryanodine receptor. Eur. Biophys. J. 2009; 39:51-9. |
| Immunogen | SPRYD5 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SPRYD5. The immunogen is located within amino acids 350 - 400 of SPRYD5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SPRYD5 antibody can be used for detection of SPRYD5 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPRYD5 |
|---|---|
| Alternative Names | SPRYD5 Antibody: SPRYD5 TRIM51A SPRYD5 Tripartite motif-containing protein 51 SPRY domain-containing protein 5 |
| Tissue Specificity | SPRYD5 antibody is predicted to not cross-react with other SPRYD protein family members. At least two isoforms of SPRYD5 are known to exist; this antibody will detect both isoforms |
Application
Immunofluorescence of SPRYD5 in mouse brain tissue with SPRYD5 antibody at 20 μg/mL.
Application
Immunohistochemistry of SPRYD5 in mouse brain tissue with SPRYD5 antibody at 5 μg/mL.
Application
Western blot analysis of SPRYD5 in rat brain tissue lysate with SPRYD5 antibody at (A) 0.25 and (B) 0.5 μg/mL .| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | SPRYD5 antibody can be used for detection of SPRYD5 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPRYD5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPRYD5 Antibody is supplied in PBS containing 0.02% sodium azide. |
