Application
Western blot analysis of SPT1 in (A) A549 and (B) HeLa cell lysate with SUMO2/3 antibody at 1 μg/mL.Application
Immunohistochemistry of SPT1 in human lung tissue with SPT1 antibody at 5 μg/mL.| Product Name | SPT1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPT1 Antibody: HSN1, LBC1, LCB1, SPT1, SPTI, HSAN1, Serine palmitoyltransferase 1, Long chain base biosynthesis protein 1, LCB 1 |
| Product description | SPT1 Antibody: Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPT1 is the long chain base subunit 1 of mammalian serine palmitoyltransferase. SPT1 is not catalytically active but is necessary for the stabilization of the SPT2 subunit and anchoring the holoenzyme to the cytosolic face of the endoplasmic reticulum. Missense mutations in this gene have been identified in patients with hereditary sensory neuropathy type 1 (HSAN1). These mutations induce a shift in the substrate specificity of the holoenzyme, leading to the formation and accumulation of two neurotoxic sphingolipids.1) Batheja AD, Uhlinger DJ, Carton JM, et al. Characterization of serine palmitoyltransferase in normal human tissues. J. Histochem. & Cytochem. 2003; 51:687-96. |
| Immunogen | SPT1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human SPT1. The immunogen is located within amino acids 380 - 430 of SPT1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| SPT1 antibody can be used for detection of SPT1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPTLC1 |
|---|---|
| Alternative Names | SPT1 Antibody: HSN1 LBC1 LCB1 SPT1 SPTI HSAN1 Serine palmitoyltransferase 1 Long chain base biosynthesis protein 1 LCB 1 |
| Molecular Weight(MW) | Predicted: 51 kDa Observed: 51 kDa |
| Sequence Similarities | 3) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | At least two isoforms of SPT1 are known to exist; this antibody will detect only isoform a. SPT1 antibody is predicted to not cross-react with SPT2. |
Application
Western blot analysis of SPT1 in (A) A549 and (B) HeLa cell lysate with SUMO2/3 antibody at 1 μg/mL.
Application
Immunohistochemistry of SPT1 in human lung tissue with SPT1 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | SPT1 antibody can be used for detection of SPT1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SPT1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SPT1 Antibody is supplied in PBS containing 0.02% sodium azide. |
