Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.| Product Name | SPTLC1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPTLC1, HSAN, HSAN1, HSN1, LBC1, LCB1, MGC14645, SPT1, SPTI |
| Product description | Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. SPTLC1 is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in SPTLC1 gene were identified in patients with hereditary sensory neuropathy type 1. Serine palmitoyltransferase, which consists of two different subunits, is the key enzyme in sphingolipid biosynthesis. It converts L-serine and palmitoyl-CoA to 3-oxosphinganine with pyridoxal 5'-phosphate as a cofactor. The product of this gene is the long chain base subunit 1 of serine palmitoyltransferase. Mutations in this gene were identified in patients with hereditary sensory neuropathy type 1. Alternatively spliced variants encoding different isoforms have been identified.1) Bi, H., (2007) Neuropathology 27 (5), 429-433. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SPTLC1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SPTLC1 antibody can be used for detection of SPTLC1 by ELISA at 1:62500. SPTLC1 antibody can be used for detection of SPTLC1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPTLC1 |
|---|---|
| Alternative Names | SPTLC1 HSAN HSAN1 HSN1 LBC1 LCB1 MGC14645 SPT1 SPTI |
| Molecular Weight(MW) | 53 kDa |
Application
Antibody used in WB on Human Brain at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate |
|---|---|
| Application Notes | SPTLC1 antibody can be used for detection of SPTLC1 by ELISA at 1:62500. SPTLC1 antibody can be used for detection of SPTLC1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SPTLC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
