Application
Immunohistochemical analysis of paraffin - embedded human skin carcinoma (top) and breast carcinoma (bottom) showing cytoplasmic and membrane location using SRA antibody with DAB staining.Application
Western blot analysis using SRA monoclonal antibody against truncated SRA recombinant protein human ovary cancer tissue lysate and A431 cell lysate .| Product Name | SRA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Steroid receptor RNA activator protein, SRAP, SRA, STRAA1, pp7684 |
| Product description | Steroid receptor RNA activator 1 (SRA), with 237-amino acid protein (about 27 kDa), belongs to the growing family of functional non-coding RNAs. SRA was originally described as the first functional noncoding RNA able to specifically coactivate the activity of steroid receptors. Specifically, SRA exists as both an RNA transcript that forms a complex with steroid receptor coactivator-1 and as a stably expressed protein. Its expression is strongly up-regulated in many human tumors of the breast, uterus, and ovary, suggesting a potential role in pathogenesis. Although coactivation of steroid-dependent transcription by SRA is accompanied by a proliferative response, overexpression is not in itself sufficient to induce turmorigenesis.1) Rainer B. Lanz, Steven S. Chua, Niall Barron. Mol. Cell. Biol, Oct 2003; 23: 7163 - 7176. |
| Immunogen | Ni-NTA purified truncated recombinant SRA expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAIHCWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000 Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | SRA1 |
|---|---|
| Alternative Names | Steroid receptor RNA activator protein SRAP SRA STRAA1 pp7684 |
Application
Immunohistochemical analysis of paraffin - embedded human skin carcinoma (top) and breast carcinoma (bottom) showing cytoplasmic and membrane location using SRA antibody with DAB staining.
Application
Western blot analysis using SRA monoclonal antibody against truncated SRA recombinant protein human ovary cancer tissue lysate and A431 cell lysate .| Positive Control | 1) Cat. No. 1202 - A431 Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 IHC(F):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000 Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SRA monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
