Application
To detect msRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant msRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.Application
To detect msRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant msRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.| Product Name | sRank Ligand Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ODF, OPG, OPGL, RANKL, Ly109l, Trance, Opgl, Rankl, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant msRANKL (murine sRANK Ligand). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| ELISA: To detect msRANKL by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant msRANKL. Sandwich: To detect msRANKL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine sRANKL (XP-5272Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant msRANKL. Western Blot: To detect msRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant msRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Tnfsf11 |
|---|---|
| Alternative Names | ODF OPG OPGL RANKL Ly109l Trance Opgl Rankl Tumor necrosis factor ligand superfamily member 11 Osteoclast differentiation factor ODF |
Application
To detect msRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant msRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Application
To detect msRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant msRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.| Application Notes | ELISA: To detect msRANKL by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant msRANKL. Sandwich: To detect msRANKL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine sRANKL (XP-5272Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant msRANKL. Western Blot: To detect msRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant msRANKL is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | sRank Ligand antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
