Application
Immunohistochemistry of sRANK-L in human liver tissue with sRANK-L antibody at 5 μg/mL.Application
Western blot analysis of sRANK-L in rat liver tissue lysate with sRANK-L antibody at (A) 0.25 and (B) 0.5 μg/mL.| Product Name | sRANK Ligand Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | sRANK Ligand Antibody: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF |
| Product description | sRANK Ligand Antibody: The receptor activator of NF-κB ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis. This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.1) Wong BR, Rho J, Arron J, et al. TRANCE is a novel ligand of the tumor necrosis factor receptor family that activates c-Jun N-terminal kinase in T cells. J. Biol. Chem. 1997; 272:25190-4. |
| Immunogen | sRANK-L antibody was raised against a 14 amino acid synthetic peptide from near the center of human sRANK-L. The immunogen is located within amino acids 140 - 190 of sRANK Ligand. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| sRANK-L antibody can be used for detection of sRANK-L by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TNFSF11 |
|---|---|
| Alternative Names | sRANK Ligand Antibody: ODF OPGL sOdf CD254 OPTB2 RANKL TRANCE hRANKL2 Tumor necrosis factor ligand superfamily member 11 Osteoclast differentiation factor ODF |
| Molecular Weight(MW) | 35 kDa |
| Sequence Similarities | 3) Cat. No. 10-201 - Human Liver Tissue Slide |
Application
Immunohistochemistry of sRANK-L in human liver tissue with sRANK-L antibody at 5 μg/mL.
Application
Western blot analysis of sRANK-L in rat liver tissue lysate with sRANK-L antibody at (A) 0.25 and (B) 0.5 μg/mL.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | sRANK-L antibody can be used for detection of sRANK-L by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | sRANK Ligand antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | sRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide. |
