Application
Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
SRD5A3 Antibody IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in 293,LNCaP,PC-3,NCI-H292 cell line lysates (35ug/lane).| Product Name | SRD5A3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Polyprenol reductase, 3-oxo-5-alpha-steroid 4-dehydrogenase 3, Steroid 5-alpha-reductase 2-like, Steroid 5-alpha-reductase 3, S5AR 3, SR type 3, SRD5A3, SRD5A2L |
| Product description | SRD5A3 converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT).1) Uemura, M., et al. Cancer Sci. 99(1):81-86(2008) |
| Immunogen | This SRD5A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 283-311 amino acids from the C-terminal region of human SRD5A3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SRD5A3 |
|---|---|
| Alternative Names | Polyprenol reductase 3-oxo-5-alpha-steroid 4-dehydrogenase 3 Steroid 5-alpha-reductase 2-like Steroid 5-alpha-reductase 3 S5AR 3 SR type 3 SRD5A3 SRD5A2L |
| Molecular Weight(MW) | 37 kDa |
Application
Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
SRD5A3 Antibody IHC analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in 293,LNCaP,PC-3,NCI-H292 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
