Application
Immunohistochemistry of SREBF2 in human spleen tissue with SREBF2 antibody at 5 μg/mL.Application
Western blot analysis of SREBF2 in PC-3 cell lysate with SREBF1 antibody at 1 μg/ml.Application
Immunofluorescence of SREBF2 in human spleen tissue with SREBF2 antibody at 20 μg/mL.| Product Name | SREBF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SREBF2 Antibody: SREBP2, bHLHd2, SREBP-2, BHLHD2, SREBP2, Sterol regulatory element-binding protein 2, Class D basic helix-loop-helix protein 2 |
| Product description | The sterol regulatory element binding transcription factor 2 (SREBF2) is a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis (1,2). The related protein SREBF1 also binds SRE1 and activates transcription in an additive fashion to SREBF2 (2). SREBF2 has been more closely associated with cholesterol synthesis and accumulation, while the SREBF1 proteins are important in the regulation of genes involved in lipid metabolism (3).1) Hua X, Yokoyama C, Wu J, et al. SREBP-2, a second basic-helix-loop-helix-leucine zipper protein that stimulates transcription by binding to a sterol regulatory element. Proc. Natl. Acad. Sci. USA 1993; 90:11603-7. |
| Immunogen | SREBF2 antibody was raised against a 15 amino acid peptide near the center of human SREBF2. The immunogen is located within amino acids 440 - 490 of SREBF2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SREBF2 antibody can be used for detection of SREBF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SREBF2 |
|---|---|
| Alternative Names | SREBF2 Antibody: SREBP2 bHLHd2 SREBP-2 BHLHD2 SREBP2 Sterol regulatory element-binding protein 2 Class D basic helix-loop-helix protein 2 |
| Molecular Weight(MW) | Predicted: 126 kDa Observed: 128 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | SREBF2 antibody is human and mouse reactive. At least three isoforms of SREBF2 are known to exist. SREBF2 antibody is predicted not to cross-react with SREBF1. |
Application
Immunohistochemistry of SREBF2 in human spleen tissue with SREBF2 antibody at 5 μg/mL.
Application
Western blot analysis of SREBF2 in PC-3 cell lysate with SREBF1 antibody at 1 μg/ml.
Application
Immunofluorescence of SREBF2 in human spleen tissue with SREBF2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1216 - PC-3 Cell Lysate |
|---|---|
| Application Notes | SREBF2 antibody can be used for detection of SREBF2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SREBF2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | SREBF2 antibody is supplied in PBS containing 0.02% sodium azide. |
