Application
Immunohistochemistry of SRPX1 in human brain tissue with SRPX1 antibody at 5 μg/mL.Application
Immunofluorescence of SRPX1 in human brain tissue with SRPX1 antibody at 20 μg/mL.Application
Western blot analysis of SRPX1 in SK-N-SH cell lysate with SRPX1 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | SRPX1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SRPX1 Antibody: DRS, ETX1, SRPX1, HEL-S-83p, Sushi repeat-containing protein SRPX |
| Product description | SRPX1 Antibody: SRPX1 was initially identified as a candidate gene for X-linked retinitis pigmentosa and is overexpressed in the trabecular network of the eye in glaucoma conditions. Its expression is significantly downregulated in a number cancer cell lines and malignant tumor tissues and its ectopic expression induces apoptosis through the endoplasmic reticulum and the activation of caspase-12, -9, and -3, indicating that it can function as a tumor suppressor. SRPX1 is also involved with the regulation of autophagy under low serum conditions and can associate with Rab24, a member of the Rab GTPase protein family that is involved in autophagy. This association of SRPX1 with Rab24 is enhanced during autophagy.1) Dry KL, Aldred MA, Edgar AJ, et al. Identification of a novel gene, ETX1 from Xp21.1, a candidate gene for X-linked retinitis pigmentosa (RP3). Hum. Mol. Genet.1995; 4:2347-53. |
| Immunogen | SRPX1 antibody was raised against a 13 amino acid synthetic peptide from near the amino terminus of human SRPX1. The immunogen is located within amino acids 30 - 80 of SRPX1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SRPX1 antibody can be used for detection of SRPX1 by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SRPX |
|---|---|
| Alternative Names | SRPX1 Antibody: DRS ETX1 SRPX1 HEL-S-83p Sushi repeat-containing protein SRPX |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of SRPX1 in human brain tissue with SRPX1 antibody at 5 μg/mL.
Application
Immunofluorescence of SRPX1 in human brain tissue with SRPX1 antibody at 20 μg/mL.
Application
Western blot analysis of SRPX1 in SK-N-SH cell lysate with SRPX1 antibody at 0.25 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | SRPX1 antibody can be used for detection of SRPX1 by Western blot at 0.25 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SRPX1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SRPX1 Antibody is supplied in PBS containing 0.02% sodium azide. |
