Application
Antibody used in WB on Human 721_B cells at 1 ug/ml.Application
Antibody used in WB on Human HepG2 at 1 ug/ml.Application
Antibody used in WB on Human MCF7 at 1 ug/ml.Application
Antibody used in WB on Human Jurkat 1 ug/ml.Application
Antibody used in WB on Human Hela at 1 ug/ml.Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Prostate at 5.0 ug/ml.| Product Name | SSX4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SSX4, MGC119056, MGC12411, CT5.4 |
| Product description | SSX4 belongs to the SSX family. It contains 1 KRAB-related domain.SSX4 could act as a modulator of transcription. The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneously humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. SSX1, SSX2 and SSX4 genes have been involved in the t (X;18) translocation characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. Chromosome Xp11 contains a segmental duplication resulting in two identical copies of synovial sarcoma, X breakpoint 4, SSX4 and SSX4B, in tail-to-tail orientation. This gene, SSX4, represents the more telomeric copy. Two transcript variants encoding distinct isoforms have been identified for this gene.1) Ayyoub, M., (2005) J. Immunol. 174 (8), 5092-5099. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SSX4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| SSX4 antibody can be used for detection of SSX4 by ELISA at 1:312500. SSX4 antibody can be used for detection of SSX4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SSX4 |
|---|---|
| Alternative Names | SSX4 MGC119056 MGC12411 CT5.4 |
| Molecular Weight(MW) | 22 kDa |
Application
Antibody used in WB on Human 721_B cells at 1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.
Application
Antibody used in WB on Human MCF7 at 1 ug/ml.
Application
Antibody used in WB on Human Jurkat 1 ug/ml.
Application
Antibody used in WB on Human Hela at 1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Prostate at 5.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | SSX4 antibody can be used for detection of SSX4 by ELISA at 1:312500. SSX4 antibody can be used for detection of SSX4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store SSX4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
