Application
ST14 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ST14 antibody detected the ST14 protein (arrow).Application
Western Blot at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | ST14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Suppressor of tumorigenicity 14 protein, Matriptase, Membrane-type serine protease 1, MT-SP1, Prostamin, Serine protease 14, Serine protease TADG-15, Tumor-associated differentially-expressed gene 15 protein, ST14, PRSS14, SNC19, TADG15 |
| Product description | ST14 is an epithelial-derived, integral membrane serine protease. This protease forms a complex with the Kunitz-type serine protease inhibitor, HAI-1, and is found to be activated by sphingosine 1-phosphate. This protease has been shown to cleave and activate hepatocyte growth factor/scattering factor, and urokinase plasminogen activator, which suggest the function of this protease as an epithelial membrane activator for other proteases and latent growth factors. The expression of this protease has been associated with breast, colon, prostate, and ovarian tumors, which implicates its role in cancer invasion, and metastasis.1) Santin, A.D., et al., Cancer 98(9):1898-1904 (2003). |
| Immunogen | This ST14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 826-855 amino acids from the C-terminal region of human ST14. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ST14 |
|---|---|
| Alternative Names | Suppressor of tumorigenicity 14 protein Matriptase Membrane-type serine protease 1 MT-SP1 Prostamin Serine protease 14 Serine protease TADG-15 Tumor-associated differentially-expressed gene 15 protein ST14 PRSS14 SNC19 TADG15 |
| Molecular Weight(MW) | 95 kDa |
Application
ST14 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the ST14 antibody detected the ST14 protein (arrow).
Application
Western Blot at 1:1000 dilution + PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
