Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
ST3GAL5 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | ST3GAL5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Lactosylceramide alpha-2,3-sialyltransferase, CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase, Ganglioside GM3 synthase, ST3Gal V, ST3GalV, Sialyltransferase 9, ST3GAL5, SIAT9 |
| Immunogen | This ST3GAL5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-418 amino acids from the C-terminal region of human ST3GAL5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ST3GAL5 |
|---|---|
| Alternative Names | Lactosylceramide alpha-2 3-sialyltransferase CMP-NeuAc:lactosylceramide alpha-2 3-sialyltransferase Ganglioside GM3 synthase ST3Gal V ST3GalV Sialyltransferase 9 ST3GAL5 SIAT9 |
| Molecular Weight(MW) | 48 kDa |
Application
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
ST3GAL5 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution + Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
