Application
Immunocytochemistry of ST3gal6 in HeLa cells with ST3gal6 antibody at 5 μg/mL.Application
Western blot analysis of ST3gal6 in HeLa cell lysate with ST3gal6 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | ST3gal6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ST3gal6 Antibody: SIAT10, ST3GALVI, SIAT10, Type 2 lactosamine alpha-2, 3-sialyltransferase, ST3Gal VI, ST3GalVI |
| Product description | ST3gal6 Antibody: Sialyltransferases catalyze the transfer of sialic acid from cytidine 5-prime monophospho-N-acetylneuraminic acid (CMP-NeuAc) to terminal positions of glycoprotein and glycolipid carbohydrate groups. Terminal NeuAc residues are key determinants of carbohydrate structures, such as the sialyl-Lewis X determinants, and are widely distributed in many cell types. However, cancer cells often express more heavily sialylated glycans on their cell surface and this feature sometimes correlates with invasiveness. In contrast, expression of ST3gal6, a member of the sialyltransferase family that sialylates type II lactosamine structures on glycoproteins and glycolipids, was found to be significantly decreased by hypermethylation of the gene in gastrointestinal cancer. At least three isoforms of ST3gal6 are known to exist.1) Dall’Olio F and Chiricolo M. Sialyltransferases in cancer. Glycoconj. J.2001; 18:841-50. |
| Immunogen | ST3gal6 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human ST3gal6. The immunogen is located within amino acids 230 - 280 of ST3gal6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCWB |
| ST3gal6 antibody can be used for detection of ST3gal6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ST3GAL6 |
|---|---|
| Alternative Names | ST3gal6 Antibody: SIAT10 ST3GALVI SIAT10 Type 2 lactosamine alpha-2 3-sialyltransferase ST3Gal VI ST3GalVI |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of ST3gal6 in HeLa cells with ST3gal6 antibody at 5 μg/mL.
Application
Western blot analysis of ST3gal6 in HeLa cell lysate with ST3gal6 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ST3gal6 antibody can be used for detection of ST3gal6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ST3gal6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ST3gal6 Antibody is supplied in PBS containing 0.02% sodium azide. |
