Application
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ST5 antibody detected the ST5 protein (arrow).| Product Name | ST5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Suppression of tumorigenicity 5 protein, DENN domain-containing protein 2B, HeLa tumor suppression 1, ST5, DENND2B, HTS1 |
| Product description | ST5 was identified by its ability to suppress the tumorigenicity of Hela cells in nude mice. The protein contains a C-terminal region that shares similarity with the Rab 3 family of small GTP binding proteins. This protein preferentially binds to the SH3 domain of c-Abl kinase, and acts as a regulator of MAPK1/ERK2 kinase, which may contribute to its ability to reduce the tumorigenic phenotype in cells.1) Majidi, M., et al., J. Biol. Chem. 275(9):6560-6565 (2000). |
| Immunogen | This ST5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1106-1137 amino acids from the C-terminal region of human ST5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ST5 |
|---|---|
| Alternative Names | Suppression of tumorigenicity 5 protein DENN domain-containing protein 2B HeLa tumor suppression 1 ST5 DENND2B HTS1 |
| Molecular Weight(MW) | 126 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the ST5 antibody detected the ST5 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
