Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ST8SIA4 Antibody in HL-60 cell line lysates (35ug/lane) Western blot analysis of ST8SIA4 Antibody in mouse spleen tissue lysates (35ug/lane)Application
ST8SIA4 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | ST8SIA4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CMP-N-acetylneuraminate-poly-alpha-2,8-sialyltransferase, 2499-, Alpha-2,8-sialyltransferase 8D, Polysialyltransferase-1, Sialyltransferase 8D, SIAT8-D, Sialyltransferase St8Sia IV, ST8SiaIV, ST8SIA4, PST, PST1, SIAT8D |
| Product description | The protein encoded by this gene catalyzes the polycondensation of alpha-2,8-linked sialic acid required for the synthesis of polysialic acid, a modulator of the adhesive properties of neural cell adhesion molecule (NCAM1). The encoded protein, which is a member of glycosyltransferase family 29, is a type II membrane protein that may be present in the Golgi apparatus.1) Johnson, M.P., et al. Hum. Genet. 126(5):655-666(2009) |
| Immunogen | This ST8SIA4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-214 amino acids from the Central region of human ST8SIA4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ST8SIA4 |
|---|---|
| Alternative Names | CMP-N-acetylneuraminate-poly-alpha-2 8-sialyltransferase 2499- Alpha-2 8-sialyltransferase 8D Polysialyltransferase-1 Sialyltransferase 8D SIAT8-D Sialyltransferase St8Sia IV ST8SiaIV ST8SIA4 PST PST1 SIAT8D |
| Molecular Weight(MW) | 41 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ST8SIA4 Antibody in HL-60 cell line lysates (35ug/lane) Western blot analysis of ST8SIA4 Antibody in mouse spleen tissue lysates (35ug/lane)
Application
ST8SIA4 Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
