Application
Western blot analysis of STIM2 in A-20 cell lysate with STIM2 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of STIM2 in Human Spleen cells with STIM2 antibody at 20 μg/mL.Application
Immunohistochemistry of STIM2 in human spleen tissue with STIM2 antibody at 2.5 μg/mL.| Product Name | STIM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | STIM2 Antibody: KIAA1482, Stromal interaction molecule 2 |
| Product description | STIM2 Antibody: In T lymphocytes, the sole pathway for Ca++ entry following antigen-receptor binding is through store-operated Ca++-release-activated Ca++ (CRAC) channels. These channels are made up of the pore-forming subunit ORAI1 and the stromal interaction molecule 1 (STIM1), a protein that functions as a Ca++ sensor and activates the CRAC channels, migrating to the plasma membrane from endoplasmic reticulum (ER)-like sites which act as the Ca++ store. A related molecule, STIM2, acts to inhibit the STIM1-mediated store-operated Ca++ entry, and can form complexes with STIM1, suggesting they may play a coordinated role in controlling Ca++ entry. At least three isoforms of STIM2 are known to exist. This STIM2 antibody is predicted to have no cross-reactivity to STIM1.1) Luik RM and Lewis RS. New insights into the molecular mechanisms of store-operated Ca 2+ signaling in T cells. Trends Mol. Med.2007; 13:103-7. |
| Immunogen | STIM2 antibody was raised against a 18 amino acid synthetic peptide from near the carboxy terminus of human STIM2. The immunogen is located within the last 50 amino acids of STIM2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| STIM2 antibody can be used for detection of STIM2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STIM2 |
|---|---|
| Alternative Names | STIM2 Antibody: KIAA1482 Stromal interaction molecule 2 |
| Sequence Similarities | 3) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Western blot analysis of STIM2 in A-20 cell lysate with STIM2 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of STIM2 in Human Spleen cells with STIM2 antibody at 20 μg/mL.
Application
Immunohistochemistry of STIM2 in human spleen tissue with STIM2 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1288 - A20 Cell Lysate |
|---|---|
| Application Notes | STIM2 antibody can be used for detection of STIM2 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | STIM2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | STIM2 Antibody is supplied in PBS containing 0.02% sodium azide. |
