Application
Western blot analysis of (A) 5 and (B) 25ng of StrepII-tagged recombinant protein with StrepII antibody at 1 μg/mL.| Product Name | StrepII-tag Antibody |
|---|---|
| Antibody Type | Tags Antibodies |
| Antigen Alias | StrepII-tag Antibody: |
| Product description | StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.1) Schmidt TGM and Skerra A. The random peptide-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Eng.1993; 6:109-22. |
| Immunogen | StrepII-tag antibody was raised against the amino acid sequence "asWSHPQFEKga". |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| StrepII-tag antibody can be used for the detection of StrepII-tagged proteins by Western blot at 1 μg/mL.: |
|
| Species Reactivity | Tag |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | StrepII-tag Antibody: |
|---|
Application
Western blot analysis of (A) 5 and (B) 25ng of StrepII-tagged recombinant protein with StrepII antibody at 1 μg/mL.| Application Notes | StrepII-tag antibody can be used for the detection of StrepII-tagged proteins by Western blot at 1 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | StrepII-tag antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide. |
