Application
Western blot analysis using STYK1 monoclonal antibody against truncated STYK1 - His recombinant protein and STYK1 (aa47 - 422) / hIgGFc transfected CHO - K1 cell lysate.Application
Immunohistochemical analysis of paraffin - embedded human ovary carcinoma (top) and human normal cerebrum (bottom) showing cytoplasmic location using STYK1 / NOK antibody with DAB staining.| Product Name | STYK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NOK, Novel oncogene with kinase domainNOK, SuRTK106 |
| Product description | Protein kinases (PKs) represent a well studied but most diverse protein superfamily. The covalent, reversible linkage of phosphate to serine, threonine, and tyrosine residues of substrate proteins by protein kinases is probably ubiquitous cellular mechanism for regulation of physiological processes. It is known to us that most signaling pathways impinge at some point on protein kinases. Here we report a human putative receptor protein kinase cDNA STYK1. The STYK1 cDNA is 2749 base pairs in length and contains an open reading frame encoding 422 amino acids. The STYK1 gene is mapped to human chromosome 12p13 and 11 exons were found. RT-PCR showed that STYK1 is widely expressed in human tissues.1) Liu L, Yu XZ and Li TS, et al. Mol Biol Rep. 2003, Jun, 30(2):91-6. |
| Immunogen | Ni-NTA purified truncated recombinant STYK1-His is expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | STYK1 |
|---|---|
| Alternative Names | NOK Novel oncogene with kinase domainNOK SuRTK106 |
Application
Western blot analysis using STYK1 monoclonal antibody against truncated STYK1 - His recombinant protein and STYK1 (aa47 - 422) / hIgGFc transfected CHO - K1 cell lysate.
Application
Immunohistochemical analysis of paraffin - embedded human ovary carcinoma (top) and human normal cerebrum (bottom) showing cytoplasmic location using STYK1 / NOK antibody with DAB staining.| Application Notes | Western Blot:1:500 - 1:2,000 IHC(P):1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | STYK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
