Application
Western blot analysis of lysates from A431, K562, CEM cell line and rat brain tissue lyaste(from left to right), using SUV39H2 Antibody (K315) at 1:1000 at each lane.Application
Western blot analysis of anti-hSUV39H2-K315 Pab (Cat. AP1281b) in 293 and HL60 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | SUV39H2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Histone-lysine N-methyltransferase SUV39H2, Histone H3-K9 methyltransferase 2, H3-K9-HMTase 2, Lysine N-methyltransferase 1B, Suppressor of variegation 3-9 homolog 2, Su(var)3-9 homolog 2, SUV39H2, KMT1B |
| Product description | The murine gene Suv39h2 encodes an H3 histone methyltransferase (HMTase) 59% identical in sequence to mouse Suv39h1. During embryogenesis, both proteins overlap in tissue expression, yet Suv39h2 transcripts are restricted to the testes in adult animals. Immunolocalization of the Suv39h2 protein during spermatogenesis indicates enrichment at the heterochromatin from the leptotene to the round spermatid stage. Moreover, Suv39h2 specifically accumulates with chromatin of the sex chromosomes, which undergo transcriptional silencing during the first meiotic prophase. Suv39h2 HMTase may also organize meiotic heterochromatin with the potential for epigenetic imprint to the male germline.1) Ota, T., et al., Nat. Genet. 36(1):40-45 (2004). |
| Immunogen | This SUV39H2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-390 amino acids from the C-terminal region of human SUV39H2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Gene Symbol | SUV39H2 |
|---|---|
| Alternative Names | Histone-lysine N-methyltransferase SUV39H2 Histone H3-K9 methyltransferase 2 H3-K9-HMTase 2 Lysine N-methyltransferase 1B Suppressor of variegation 3-9 homolog 2 Su(var)3-9 homolog 2 SUV39H2 KMT1B |
| Molecular Weight(MW) | 47 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Western blot analysis of lysates from A431, K562, CEM cell line and rat brain tissue lyaste(from left to right), using SUV39H2 Antibody (K315) at 1:1000 at each lane.
Application
Western blot analysis of anti-hSUV39H2-K315 Pab (Cat. AP1281b) in 293 and HL60 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
