Application
Western blot analysis of SWAP70 using rabbit polyclonal SWAP70 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SWAP70 gene (Lane 2).Application
Western blot analysis of anti-SWAP70 Antibody in HepG2 cell line lysates (35ug/lane). SWAP70 was detected using the purified polyclonal antibody.| Product Name | SWAP70 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SWAP70 is a phosphatidylinositol 3,4,5-trisphosphate-dependent guanine nucleotide exchange factor (GEF) which, independently of RAS, transduces signals from tyrosine kinase receptors to RAC. It also mediates signaling of membrane ruffling. This protein regulates the actin cytoskeleton as an effector or adapter protein in response to agonist stimulated phosphatidylinositol (3,4)-bisphosphate production and cell protrusion.1) Hilpel, et al., Mol. Biol. Cell 14(8):3242-3253 (2003). |
| Immunogen | This SWAP70 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-256 amino acids from the Central region of human SWAP70. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | SWAP70 |
|---|---|
| Alternative Names | Switch-associated protein 70 SWAP-70 SWAP70 KIAA0640 |
| Molecular Weight(MW) | 69 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of SWAP70 using rabbit polyclonal SWAP70 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the SWAP70 gene (Lane 2).
Application
Western blot analysis of anti-SWAP70 Antibody in HepG2 cell line lysates (35ug/lane). SWAP70 was detected using the purified polyclonal antibody.| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
