Application
Western blot analysis of extract from rat brain and mouse brain using synaptophysin 2 Antibody.| Product Name | synaptophysin 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Synpr |
| Product description | Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms, and thus can be used as a marker in tumor diagnosis (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).1) Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4. Valtorta, F. et al. (2004) Bioessays 26, 445-53. Arthur, C. P. and Stowell, M. H. (2007) Structure 15, 707-14. Bonanomi, D. et al. (2007) Biochem J 404, 525-34. |
| Immunogen | synaptophysin 2 antibody was raised against a peptide sequence around aa.257~261 (G-P-T-S-F) derived from Rat synaptophysin 2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Synpr |
|---|---|
| Alternative Names | Synpr |
| Molecular Weight(MW) | 37 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total synaptophysin 2 protein. |
| Entrez Gene | 66030 |
|---|---|
| Protein Accession | NP_076464.1 |
Application
Western blot analysis of extract from rat brain and mouse brain using synaptophysin 2 Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
