Application
Western blot analysis of lysed extracts from HeLa cells using Synaptotagmin 1/2 (Ab-202/199).Application
Immunofluorescence staining of methanol-fixed HeLa cells using Synaptotagmin 1/2 (Ab-202/199).| Product Name | Synaptotagmin 1 / 2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P65, SYT, SVP65, SYT1/SYT2 |
| Product description | The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin I participates in triggering neurotransmitter release at the synapse1) Gustavsson N, et al. Proc Natl Acad Sci U S A. 2008 Mar 11; 105 (10): 3992-7. Cnops L, et al. Cereb Cortex. 2008 May; 18 (5): 1221-31. Lynch KL, et al. Mol Biol Cell. 2007 Dec; 18 (12): 4957-68. |
| Immunogen | Synaptotagmin 1/2 (Ab-202/199) antibody was raised against a peptide sequence around aa.200~204/197~201 (R-K-T-L-N) derived from Human. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYT1 AgaP_AGAP008122 |
|---|---|
| Alternative Names | P65 SYT SVP65 SYT1/SYT2 |
| Molecular Weight(MW) | 46 kDa |
| Tissue Specificity | This antibody detects endogenous level of total Synaptotagmin 1/2 protein. |
| Entrez Gene | 6857 / 127833 |
|---|---|
| Protein Accession | NP_001129277.1 / NP_001129976.1 |
Application
Western blot analysis of lysed extracts from HeLa cells using Synaptotagmin 1/2 (Ab-202/199).
Application
Immunofluorescence staining of methanol-fixed HeLa cells using Synaptotagmin 1/2 (Ab-202/199).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
