Application
Western blot analysis in U251,A549 and MDA-MB453 cell line lysates (35ug/lane).Application
SYNCI Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYNCI Antibody (N-term)for immunohistochemistry.Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | SYNCI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Syncoilin, Syncoilin intermediate filament 1, Syncoilin-1, SYNC, SYNC1 |
| Product description | This gene encodes a member of the intermediate filament family which contains an N-terminal head domain, followed by a central coiled-coil region and a short C-terminal tail. The protein is highly expressed in skeletal and cardiac muscle. The protein links the dystrophin associated protein complex (DAPC) to desmin filaments in muscle and may have a structural role in striated muscle. Multiple transcript variants encoding different isoforms have been found for this gene.1) Wakayama, Y., et al. Int. J. Neurosci. 120(2):144-149(2010) |
| Immunogen | This SYNCI antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the N-terminal region of human SYNCI. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYNC |
|---|---|
| Alternative Names | Syncoilin Syncoilin intermediate filament 1 Syncoilin-1 SYNC SYNC1 |
| Molecular Weight(MW) | 55 kDa |
Application
Western blot analysis in U251,A549 and MDA-MB453 cell line lysates (35ug/lane).
Application
SYNCI Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYNCI Antibody (N-term)for immunohistochemistry.
Application
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
