Application
Western blot analysis of SYNGR3 in mouse brain tissue lysate with SYNGR3 antibody at (A) 1 and (B) 2 μg/mL .Application
Immunofluorescence of SYNGR3 in Rat Brain cells with SYNGR3 antibody at 20 μg/mL.Application
Immunohistochemistry of SYNGR3 in rat brain tissue with SYNGR3 antibody at 2.5 μg/mL.| Product Name | SYNGR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SYNGR3 Antibody: Synaptogyrin-3 |
| Product description | SYNGR3 Antibody: Synaptogyrins comprise a family of tyrosine-phosphorylated membrane proteins with two neuronal (SYNGR1 and SYNGR3) and one ubiquitous (SYNGR2) members. SYNGR1 and -3 are synaptic vesicle proteins, residing in some cases on the same synaptic vesicle, and are thought to be involved in the regulation of neurotransmitter release. SYNGR2, by contrast, is absent from synaptic vesicles. The role and localization of a fourth synaptogyrin, SYNGR4, is unclear. SYNGR3 is predominantly expressed in brain and placenta. SYNGR3 is involved in the positive regulation of dopamine transporter activity and facilitates the physical and functional interactions of the transporter with the dopamine vesicular storage system, allowing a more efficient loading of the vesicles with extracellular dopamine after release.1) Belizaire R, Komanduri C, Wooten K, et al. Characterization of synaptogyrin 3 as a new synaptic vesicle protein. J. Comp. Neurol.2004; 470:266-81. |
| Immunogen | SYNGR3 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human SYNGR3. The immunogen is located within the last 50 amino acids of SYNGR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SYNGR3 antibody can be used for detection of SYNGR3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYNGR3 |
|---|---|
| Alternative Names | SYNGR3 Antibody: Synaptogyrin-3 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (89%) |
Application
Western blot analysis of SYNGR3 in mouse brain tissue lysate with SYNGR3 antibody at (A) 1 and (B) 2 μg/mL .
Application
Immunofluorescence of SYNGR3 in Rat Brain cells with SYNGR3 antibody at 20 μg/mL.
Application
Immunohistochemistry of SYNGR3 in rat brain tissue with SYNGR3 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | SYNGR3 antibody can be used for detection of SYNGR3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SYNGR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SYNGR3 Antibody is supplied in PBS containing 0.02% sodium azide. |
