Synaptojanin 1 is a phosphoinositide phosphatase that regulates levels of membrane phosphatidylinositol-4,5-bisphosphate. As such, expression of this enzyme may affect synaptic transmission and membrane trafficking.Synaptojanin-1 is a phosphatidylinositol (4,5)-bisphosphate (PI (4,5)P2) that has a role in clathrin-coated pit dynamics Perera et al. (2006) [PubMed 17158794].1) Thole, J.M., (2008) Plant Cell 20 (2), 381-395.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SYNJ1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
SYNJ1 antibody can be used for detection of SYNJ1 by ELISA at 1:62500. SYNJ1 antibody can be used for detection of SYNJ1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
SYNJ1 antibody can be used for detection of SYNJ1 by ELISA at 1:62500. SYNJ1 antibody can be used for detection of SYNJ1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SYNJ1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.