WB
Various lysates were subjected to SDS PAGE followed by western blot with SYNJ1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.WB
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with SYNJ1 Antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using SYNJ1 Antibody at dilution of 1:200 (under 10x lens).IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using SYNJ1 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using SYNJ1 antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | SYNJ1 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human SYNJ1 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:50-1:500 ICC/IF:1:20-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.7mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYNJ1 |
|---|---|
| Gene Synonyms | DEE53 EIEE53 INPP5G PARK20 |
| Gene Full Name | synaptojanin 1 |
| Gene Summary | This gene encodes a phosphoinositide phosphatase that regulates levels of membrane phosphatidylinositol-4,5-bisphosphate. As such, expression of this enzyme may affect synaptic transmission and membrane trafficking. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] |
| Alternative Names | EC:3.1.3.36 INPP5G KIAA0910 Synaptic inositol 1 4 5-trisphosphate 5-phosphatase 1 synaptojanin 1 |
| Molecular Weight(MW) | 140kDa |
| Cellular Localization | Cytoplasm. |
WB
Various lysates were subjected to SDS PAGE followed by western blot with SYNJ1 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with SYNJ1 Antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using SYNJ1 Antibody at dilution of 1:200 (under 10x lens).
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using SYNJ1 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (-20℃ Ethanol) fixed NIH/3T3 cells using SYNJ1 antibody at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:5000 IHC:1:50-1:500 ICC/IF:1:20-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
