Application
Western blot analysis of SYNPO2 in human skeletal muscle tissue lysate with SYNPO2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of SYNPO2 in mouse skeletal muscle tissue with SYNPO2 antibody at 5 μg/mL.Application
Immunohistochemistry of SYNPO2 in mouse skeletal muscle tissue with SYNPO2 antibody at 20 μg/mL.| Product Name | SYNPO2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | SYNPO2 Antibody: SYNPO2 was initially identified as myopodin, a member of the synaptopodin family that contains one PPXY motif and multiple PXXP motifs. It colocalizes with alpha-actinin and is found at the Z-disc and during stress conditions will translocate to the nucleus, suggesting that it is part of signaling pathways in addition to its function as a structural protein. SYNPO2 has been shown to bind to calmodulin, alpha-actinin, and smooth muscle myosin and will stimulate actin polymerization in a calmodulin dependent manner, consistent with its proposed role in organizing the cytoskeleton. While deletion of SYNPO2 has been reported to be highly correlated with the invasiveness of prostate cancers, other reports suggest that down-regulation of SYNPO2 reduces the invasiveness and motility of prostate cancer cells.1) Weins A, Schwarz K, Faul C, et al. Differentiation- and stress-dependent nuclear cytoplasmic redistribution of myopodin, a novel actin-bundling protein. J. Cell Biol.2001; 155:393-404. |
| Immunogen | SYNPO2 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human SYNPO2. The immunogen is located within amino acids 320 - 370 of SYNPO2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IF:1:100~1:500 IHC-P:1:50~1:200 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYNPO2 |
|---|---|
| Alternative Names | SYNPO2 Antibody: Synaptopodin-2 Genethonin-2 |
Application
Western blot analysis of SYNPO2 in human skeletal muscle tissue lysate with SYNPO2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of SYNPO2 in mouse skeletal muscle tissue with SYNPO2 antibody at 5 μg/mL.
Application
Immunohistochemistry of SYNPO2 in mouse skeletal muscle tissue with SYNPO2 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IF:1:100~1:500 IHC-P:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | SYNPO2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SYNPO2 Antibody is supplied in PBS containing 0.02% sodium azide. |
