Application
Western blot analysis of Syntaphilin in human brain tissue lysate with Syntaphilin antibody at (A) 2 and (B) 4 μg/mL.Application
Immunofluorescence of Syntaphilin in Human Brain cells with Syntaphilin antibody at 20 μg/mL.Application
Immunohistochemistry of Syntaphilin in human brain with Syntaphilin antibody at 5 μg/mL.| Product Name | Syntaphilin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Syntaphilin Antibody: Syntaphilin was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function. Multiple isoforms are known to exist.1) Lao G, Scheuss V, Gerwin CM, et al. Syntaphilin: a syntaxin-1 clamp that controls SNARE assembly. Neuron2000; 25:191-201. |
| Immunogen | Syntaphilin antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human Syntaphilin. The immunogen is located within amino acids 80 - 130 of Syntaphilin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 2 - 4 μg/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SNPH |
|---|---|
| Alternative Names | Syntaphilin Antibody: bA314N13.5 KIAA0374 Syntaphilin |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of Syntaphilin in human brain tissue lysate with Syntaphilin antibody at (A) 2 and (B) 4 μg/mL.
Application
Immunofluorescence of Syntaphilin in Human Brain cells with Syntaphilin antibody at 20 μg/mL.
Application
Immunohistochemistry of Syntaphilin in human brain with Syntaphilin antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1303 - Human Brain Tissue Lysate |
|---|---|
| Application Notes | Syntaphilin antibody can be used for detection of Syntaphilin by Western blot at 2 - 4 μg/mL. Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | Syntaphilin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Syntaphilin Antibody is supplied in PBS containing 0.02% sodium azide. |
