Application
Western blot analysis of SYPL2 in human spleen tissue lysate with SYPL2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of SYPL2 in Human Spleen cells with SYPL2 antibody at 20 μg/mL.Application
Immunohistochemistry of SYPL2 in human spleen tissue with SYPL2 antibody at 2.5 μg/mL.| Product Name | SYPL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SYPL2 Antibody: MG29, Synaptophysin-like protein 2 |
| Product description | SYPL2 Antibody: SYPL2, also known as Mitsugumin 29, was initially identified as a transmembrane protein from the triad junction in skeletal muscle that had significant homology with members of the synaptophysin family. SYPL2 is thought to participate in the excitation-contraction coupling process of skeletal muscle as SYPL2-null mice showed reduced muscle contractile force and altered triad junction structure and increased susceptibility to fatigue of the skeletal muscle. SYPL2 plays a critical role in muscle Ca2+ signaling by regulating the process of store-operated Ca2+ entry and interacts with ryanodine receptor (RyR), thereby influencing intracellular Ca2+ homeostasis through changes in the RyR/Ca2+ release function. Co-expression of SYPL2 and RyR in cultured cells leads to apoptotic cell death resulting from the depletion of Ca2+ from the intracellular stores.1) Takeshima H, Simuta M, Komazaki S, et al. Mitsugumin29, a novel synaptophysin family member from the triad junction in skeletal muscle. Biochem. J.1998; 331:317-22. |
| Immunogen | SYPL2 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human SYPL. The immunogen is located within the last 50 amino acids of SYPL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| SYPL2 antibody can be used for detection of SYPL2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SYPL2 |
|---|---|
| Alternative Names | SYPL2 Antibody: MG29 Synaptophysin-like protein 2 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | At least two isoforms of SYPL2 are known to exist. SYPL2 antibody will not cross-react with SYPL1. |
| Entrez Gene | 284612 |
|---|---|
| Protein Accession | NP_001035799 |
Application
Western blot analysis of SYPL2 in human spleen tissue lysate with SYPL2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of SYPL2 in Human Spleen cells with SYPL2 antibody at 20 μg/mL.
Application
Immunohistochemistry of SYPL2 in human spleen tissue with SYPL2 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | SYPL2 antibody can be used for detection of SYPL2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | SYPL2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | SYPL2 Antibody is supplied in PBS containing 0.02% sodium azide. |
