SYT3 may be involved in Ca (2+)-dependent exocytosis of secretory vesicles through Ca (2+) and phospholipid binding to the C2 domain or may serve as Ca (2+) sensors in the process of vesicular trafficking and exocytosis.1) Mehrle, A., Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SYT3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
SYT3 antibody can be used for detection of SYT3 by ELISA at 1:62500. SYT3 antibody can be used for detection of SYT3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on mouse, rat, and human brain at 1 ug/ml.
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Positive Control
1) 293T Cell Lysate
Application Notes
SYT3 antibody can be used for detection of SYT3 by ELISA at 1:62500. SYT3 antibody can be used for detection of SYT3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store SYT3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.