Application
Western blot analysis of T-cadherin in 3T3 lysate with T-cadherin antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunohistochemistry of T cadherin in mouse brain tissue with T cadherin Antibodyat 5 μg/mL.Application
Immunofluorescence of T cadherin in mouse brain tissue with T cadherin Antibodyat 20 μg/mL.Application
Immunofluorescence of T-cadherin in K562 cells with T-cadherin antibody at 20 μg/mL.| Product Name | T-cadherin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | T-cadherin Antibody: T-cadherin was initially identified as cadherin-type cell adhesion molecule expressed in various neuronal populations in a temporally and spatially restricted pattern during axon growth. T-cadherin is an atypical member of the cadherin family because it does not possess the typical transmembrane and cytoplasmic domains but is instead anchored to the plasma membrane by glycosylphosphatidylinositol (GPI) linkage. T-cadherin may play a role in malignant tumor development as loss of the chromosome locus containing the T-cadherin gene correlates with the development of a variety of cancers. Recently it has been shown that T-cadherin can act as a receptor for hexameric and high-molecular weight forms of adiponectin, suggesting that T-cadherin may also play a role in metabolic regulation.1) Ranscht B and Dours-Zimmerman MT. T-cadherin, a novel cadherin cell adhesion molecule in the nervous system lacks the conserved cytoplasmic region. Neuron 1991; 7:391-402. |
| Immunogen | T-cadherin antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human T-cadherin. The immunogen is located within amino acids 150 - 200 of T-cadherin. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| T-cadherin antibody can be used for the detection of T-cadherin by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ENTPD3 |
|---|---|
| Alternative Names | T-cadherin Antibody: HB6 CD39L3 NTPDase-3 Ectonucleoside triphosphate diphosphohydrolase 3 CD39 antigen-like 3 NTPDase 3 |
| Molecular Weight(MW) | Predicted: 78, 84 kDa Observed: 85 kDa |
| Sequence Similarities | 4) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | At least two isoforms of T-cadherin are known to exist. |
Application
Western blot analysis of T-cadherin in 3T3 lysate with T-cadherin antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunohistochemistry of T cadherin in mouse brain tissue with T cadherin Antibodyat 5 μg/mL.
Application
Immunofluorescence of T cadherin in mouse brain tissue with T cadherin Antibodyat 20 μg/mL.
Application
Immunofluorescence of T-cadherin in K562 cells with T-cadherin antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1212 - 3T3 Cell Lysate |
|---|---|
| Application Notes | T-cadherin antibody can be used for the detection of T-cadherin by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for for immunohistochemistry starting at 5 μg/mL and immunocytochemistry starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | T-cadherin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | T-cadherin Antibody is supplied in PBS containing 0.02% sodium azide. |
