Application
Western blot analysis of TACE in HeLa (A, D), Jurkat (B, E) and Raji (C,F) cell lysate in (A-C) the absence or (D-F) the presence of blocking peptide with TACE antibody at 0.5 μg/mL.Application
Immunofluorescence of TACE in HeLa cells with TACE antibody at 10 μg/mL.Application
Immunocytochemistry of TACE in HeLa cells with TACE antibody at 10 μg/mL.| Product Name | TACE Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TACE Antibody: CSVP, TACE, NISBD, ADAM18, CD156B, CSVP, Disintegrin and metalloproteinase domain-containing protein 17, Snake venom-like protease, ADAM 17 |
| Product description | TACE Antibody: Tumor-necrosis factor-alpha is a proinflammatory cytokine and contributes to a variety of inflammatory disease responses and programmed cell death. TNF-α is synthesized as a 26K type II membrane-bound precursor that is cleaved by a convertase to generate secreted 17K mature TNF-α. TNF-α converting enzyme (TACE) protein was recently purified and the human and mouse TACE cDNAs were cloned by several groups separately. TACE is a membrane-bound metalloprotease-disintegrin in the family of mammalian ADAM (for a disintegrin and metalloprotease). TACE also processes other cell surface proteins, including TNF receptor, TGFalpha, the L-selectin adhesion molecule, and alpha-cleavage of amyloid protein precursor (APP). TACE mRNA is expressed in a variety of human and murine tissues. TACE was selected as one of the few targets in cytokine activation by the Eighth International Conference of the Inflammation Research Association.1) Black RA, Rauch CT , Kozlosky CJ, et al. A metalloproteinase disintegrin that releases tumour-necrosis factor-α from cells. Nature 1997;385:729-733 |
| Immunogen | TACE antibody was raised against a peptide corresponding to 17 amino acids near the carboxy terminus of human TACE. The immunogen is located within the last 50 amino acids of TACE. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSICCIFWB |
| TACE antibody can be used for detection of TACE by Western blot at 0.5 μg/mL. For immunocytochemistry use 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ADAM17 |
|---|---|
| Alternative Names | TACE Antibody: CSVP TACE NISBD ADAM18 CD156B CSVP Disintegrin and metalloproteinase domain-containing protein 17 Snake venom-like protease ADAM 17 |
| Molecular Weight(MW) | Predicted: 93 kDa Observed: 125 kDa |
| Sequence Similarities | 4) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE. |
Application
Western blot analysis of TACE in HeLa (A, D), Jurkat (B, E) and Raji (C,F) cell lysate in (A-C) the absence or (D-F) the presence of blocking peptide with TACE antibody at 0.5 μg/mL.
Application
Immunofluorescence of TACE in HeLa cells with TACE antibody at 10 μg/mL.
Application
Immunocytochemistry of TACE in HeLa cells with TACE antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | TACE antibody can be used for detection of TACE by Western blot at 0.5 μg/mL. For immunocytochemistry use 10 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TACE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TACE Antibody is supplied in PBS containing 0.02% sodium azide. |
