Application
Western blot analysis in Jurkat,NCI-H292 cell line lysates (35ug/lane).| Product Name | TAF10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription initiation factor TFIID subunit 10, STAF28, Transcription initiation factor TFIID 30 kDa subunit, TAF(II)30, TAFII-30, TAFII30, TAF10, TAF2A, TAF2H, TAFII30 |
| Product description | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the small subunits of TFIID that is associated with a subset of TFIID complexes. Studies with human and mammalian cells have shown that this subunit is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs.1) Egloff, S., et al. J. Biol. Chem. 285(27):20564-20569(2010) |
| Immunogen | This TAF10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 121-150 amino acids from the Central region of human TAF10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAF10 |
|---|---|
| Alternative Names | Transcription initiation factor TFIID subunit 10 STAF28 Transcription initiation factor TFIID 30 kDa subunit TAF(II)30 TAFII-30 TAFII30 TAF10 TAF2A TAF2H TAFII30 |
| Molecular Weight(MW) | 22 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western blot analysis in Jurkat,NCI-H292 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
