Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in NCI-H292,Jurkat cell line and mouse liver lysates (15ug/lane).Application
TAF1D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | TAF1D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TATA box-binding protein-associated factor RNA polymerase I subunit D, RNA polymerase I-specific TBP-associated factor 41 kDa, TAFI41, TATA box-binding protein-associated factor 1D, TBP-associated factor 1D, Transcription initiation factor SL1/TIF-IB subun |
| Product description | TAF1D is a member of the SL1 complex, which includes TBP (MIM 600075) and TAF1A (MIM 604903), TAF1B (MIM 604904), and TAF1C (MIM 604905), and plays a role in RNA polymerase I transcription (Wang et al., 2004 [PubMed 15520167]; Gorski et al., 2007 [PubMed 17318177]).1) Gorski, J.J., et al. EMBO J. 26(6):1560-1568(2007) |
| Immunogen | This TAF1D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human TAF1D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAF1D |
|---|---|
| Alternative Names | TATA box-binding protein-associated factor RNA polymerase I subunit D RNA polymerase I-specific TBP-associated factor 41 kDa TAFI41 TATA box-binding protein-associated factor 1D TBP-associated factor 1D Transcription initiation factor SL1/TIF-IB subun |
| Molecular Weight(MW) | 32 kDa |
Application
Flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in NCI-H292,Jurkat cell line and mouse liver lysates (15ug/lane).
Application
TAF1D Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
