Application
Western blot analysis of lysates from HT-29, Jurkat, KG-1, PC-3 cell line (from left to right), using TAF2 Antibody at 1:1000 at each lane.Application
TAF2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TAF2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription initiation factor TFIID subunit 2, 150 kDa cofactor of initiator function, RNA polymerase II TBP-associated factor subunit B, TBP-associated factor 150 kDa, Transcription initiation factor TFIID 150 kDa subunit, TAF(II)150, TAFII-150, TAFII15 |
| Product description | Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that is stably associated with the TFIID complex. It contributes to interactions at and downstream of the transcription initiation site, interactions that help determine transcription complex response to activators.1) Olsen, J.V., et al. Cell 127(3):635-648(2006) |
| Immunogen | This TAF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1152-1180 amino acids from the C-terminal region of human TAF2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAF2 |
|---|---|
| Alternative Names | Transcription initiation factor TFIID subunit 2 150 kDa cofactor of initiator function RNA polymerase II TBP-associated factor subunit B TBP-associated factor 150 kDa Transcription initiation factor TFIID 150 kDa subunit TAF(II)150 TAFII-150 TAFII15 |
| Molecular Weight(MW) | 137 kDa |
Application
Western blot analysis of lysates from HT-29, Jurkat, KG-1, PC-3 cell line (from left to right), using TAF2 Antibody at 1:1000 at each lane.
Application
TAF2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
