Application
Western Blot at 1:1000 dilution + Ramos whole cell lysate Lysates/proteins at 20 ug per lane.Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TAF4B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription initiation factor TFIID subunit 4B, Transcription initiation factor TFIID 105 kDa subunit, TAF(II)105, TAFII-105, TAFII105, TAF4B, TAF2C2, TAFII105 |
| Product description | TATA-binding protein associated factors (TAFs) participate, with TATA binding protein (TBP; MIM 600075), in the formation of the TFIID protein complex (see MIM 313650), which is involved in the initiation of gene transcription by RNA polymerase II (see MIM 180660).1) Kalogeropoulou, M., et al. Mol. Cancer Res. 8(4):554-568(2010) |
| Immunogen | This TAF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 647-675 amino acids from the C-terminal region of human TAF4B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAF4B |
|---|---|
| Alternative Names | Transcription initiation factor TFIID subunit 4B Transcription initiation factor TFIID 105 kDa subunit TAF(II)105 TAFII-105 TAFII105 TAF4B TAF2C2 TAFII105 |
| Molecular Weight(MW) | 91 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western Blot at 1:1000 dilution + Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
