The intronless gene for this transcription coactivator is located between the protocadherin beta and gamma gene clusters on chromosome 5. The protein encoded by this gene is a component of the TFIID protein complex, a complex which binds to the TATA box in class II promoters and recruits RNA polymerase II and other factors. This particular subunit interacts with the largest TFIID subunit, as well as multiple transcription activators. The protein is required for transcription by promoters targeted by RNA polymerase II.1) Fukuchi, J., et al., (2004) J.Biol.Chem.279(29), 29921-29929.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF7.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
TAF7 antibody can be used for detection of TAF7 by ELISA at 1:1562500. TAF7 antibody can be used for detection of TAF7 by western blot at 5.0-8.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Jurkat 5.0-8.0 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
TAF7 antibody can be used for detection of TAF7 by ELISA at 1:1562500. TAF7 antibody can be used for detection of TAF7 by western blot at 5.0-8.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TAF7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 200 μL of distilled water. Final antibody concentration is 1 mg/mL.