Application
Western blot analysis of lysates from Hela, U-251 MG cell line (from left to right), using TAF8 Antibody at 1:1000 at each lane.Application
TAF8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | TAF8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Transcription initiation factor TFIID subunit 8, Protein taube nuss, TBP-associated factor 43 kDa, TBP-associated factor 8, Transcription initiation factor TFIID 43 kDa subunit, TAFII-43, TAFII43, hTAFII43, TAF8, TAFII43, TBN |
| Product description | This gene encodes one of several TATA-binding protein (TBP)-associated factors (TAFs), which are integral subunits of the general transcription factor complex TFIID. TFIID recognizes the core promoter of many genes and nucleates the assembly of a transcription preinitiation complex containing RNA polymerase II and other initiation factors. The protein encoded by this gene contains an H4-like histone fold domain, and interacts with several subunits of TFIID including TBP and the histone-fold protein TAF10. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq].1) Ganesh, S.K., et al. Nat. Genet. 41(11):1191-1198(2009) |
| Immunogen | This TAF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 151-178 amino acids from the Central region of human TAF8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAF8 |
|---|---|
| Alternative Names | Transcription initiation factor TFIID subunit 8 Protein taube nuss TBP-associated factor 43 kDa TBP-associated factor 8 Transcription initiation factor TFIID 43 kDa subunit TAFII-43 TAFII43 hTAFII43 TAF8 TAFII43 TBN |
| Molecular Weight(MW) | 34 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of lysates from Hela, U-251 MG cell line (from left to right), using TAF8 Antibody at 1:1000 at each lane.
Application
TAF8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
