Application
Western blot analysis of TAF9 in HepG2 cell lysate with TAF9 antibody at (A) 1 and (B) 2μg/ml.Application
Immunofluorescence of TAF9 in mouse liver tissue with TAF9 antibody at 20 μg/mL.Application
Immunohistochemistry of TAF9 in mouse liver tissue with TAF9 antibody at 5 μg/mL.| Product Name | TAF9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TAF9 Antibody: TAF2G, TAFII31, TAFII32, MGC:5067, TAFII-31, TAFII-32, TAFIID32, STAF31/32, TAF2G, Transcription initiation factor TFIID subunit 9, RNA polymerase II TBP-associated factor subunit G |
| Product description | Initiation of transcription by RNA polymerase II is coordinated by the transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and the TBP-associated factors (TAFs). TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation (1). TAF9 is one of the smaller subunits of TFIID that binds to the basal transcription factor GTF2B as well as to several transcriptional activators such as p53 and VP16 (2).1) Zawel L and Reinberg D. Advances in RNA polymerase II transcription. Curr. Opin. Cell Biol. 1992; 4:488-95. |
| Immunogen | TAF9 antibody was raised against a 17 amino acid peptide near the center of human TAF9. The immunogen is located within amino acids 110 - 160 of TAF9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| TAF9 antibody can be used for detection of TAF9 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAF9 |
|---|---|
| Alternative Names | TAF9 Antibody: TAF2G TAFII31 TAFII32 MGC:5067 TAFII-31 TAFII-32 TAFIID32 STAF31/32 TAF2G Transcription initiation factor TFIID subunit 9 RNA polymerase II TBP-associated factor subunit G |
| Molecular Weight(MW) | Predicted: 29 kDa Observed: 28 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Tissue Specificity | TAF9 antibody is human, mouse and rat reactive. Multiple isoforms of TAF9 are known to exist. TAF9 is predicted to not cross-react with other TAF family members. |
Application
Western blot analysis of TAF9 in HepG2 cell lysate with TAF9 antibody at (A) 1 and (B) 2μg/ml.
Application
Immunofluorescence of TAF9 in mouse liver tissue with TAF9 antibody at 20 μg/mL.
Application
Immunohistochemistry of TAF9 in mouse liver tissue with TAF9 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | TAF9 antibody can be used for detection of TAF9 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TAF9 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TAF9 Antibody is supplied in PBS containing 0.02% sodium azide. |
