Initiation of transcription by RNA polymerase II requires
the activities of more than 70 polypeptides. The protein that
coordinates these activities is transcription factor IID (TFIID),
which binds to the core promoter to position the polymerase
properly, serves as the scaffold for assembly of the remainder of
the transcription complex, and acts as a channel for regulatory
signals. TFIID is composed of the TATA-binding protein (TBP) and a
group of evolutionarily conserved proteins known as TBP-associated
factors or TAFs. TAFs may participate in basal transcription, serve
as coactivators, function in promoter recognition or modify general
transcription factors (GTFs) to facilitate complex assembly and
transcription initiation. This gene encodes a protein that is
similar to one of the small subunits of TFIID, TBP-associated
factor 9, and is also a subunit of TFIID. TAF9 and TAF9b share some
functions but also have distinct roles in the transcriptional
regulatory process.1) Frontini, M., et al. Mol. Cell. Biol. 25(11):4638-4649(2005)
Immunogen
This TAF9B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 44-72 amino acids from the N-terminal region of human TAF9B.
Western blot analysis in MDA-MB453 cell line lysates (35ug/lane).
Application Notes
For WB starting dilution is: 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.