Application
Western blot analysis of TAK1 in Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.Application
Immunofluorescence of TAK1 in Rat Thymus cells with TAK1 antibody at 10 μg/mL.| Product Name | TAK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | TAK1 Antibody: TAK1, MEKK7, TGF1a, TAK1, Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1, TGF-beta-activated kinase 1 |
| Product description | TAK1 Antibody: TAK1 (TGF-beta activated protein kinase 1) is a widely expressed enzyme originally identified as a mitogen-activated protein kinase kinase kinase (MAP3K7). It has since been shown to mediate various intracellular signaling pathways, such as those induced by TGF-beta and members of the Toll-IL-1R (TIR) superfamily, thus acting as an intermediate in both proliferative and innate and adaptive immune responses. TAK1 is normally present in cells in a complex with TAK1-binding protein 1 (TAB1) and either TAB2 or TAB3. Association with TAB1 triggers TAK1 kinase activity by inducing TAK1 autophosphorylation, while TAB2 and possibly TAB3 may contribute to SAPK2a/p38a-mediated feedback inhibition of TAK1 activity.1) Yamaguchi K, Shirakabe K, Shibuya H, et al. Identification of a member of the MAPKKK family as a potential mediator of TGF-β signal transduction. Science 1995; 270:2008-11. |
| Immunogen | TAK1 antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino-terminus of human TAK1 isoform a. The immunogen is located within amino acids 40 - 90 of TAK1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| TAK1 antibody can be used for the detection of TAK1 by Western blot at 1 to 4 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAP3K7 |
|---|---|
| Alternative Names | TAK1 Antibody: TAK1 MEKK7 TGF1a TAK1 Mitogen-activated protein kinase kinase kinase 7 Transforming growth factor-beta-activated kinase 1 TGF-beta-activated kinase 1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Western blot analysis of TAK1 in Rat thymus cell lysate with TAK1 antibody at (A) 1, (B) 2, and (C) 4 μg/mL.
Application
Immunofluorescence of TAK1 in Rat Thymus cells with TAK1 antibody at 10 μg/mL.| Positive Control | 1) Cat. No. 1471 - Rat Thymus Tissue Lysate |
|---|---|
| Application Notes | TAK1 antibody can be used for the detection of TAK1 by Western blot at 1 to 4 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TAK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | TAK1 Antibody is supplied in PBS containing 0.02% sodium azide. |
