Application
Western blot analysis of extract from rat brain, rat hippocampus and mouse brain using Tau (phospho-Ser416).| Product Name | Tau (phospho Ser416) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | pTau, Mtapt, RNPTAU, Mapt |
| Product description | Tau is a heterogeneous microtubule-associated protein that promotes and stabilizes microtubule assembly, especially in axons. Six isoforms with different amino-terminal inserts and different numbers of tandem repeats near the carboxy-terminus have been identified, and tau is hyperphosphorylated at approximately 25 sites by ERK, GSK-3 and CDK5 (1-2). Phosphorylation decreases the ability of tau to bind to microtubules. Neurofibrillary tangles are a major hallmark of Alzheimer's disease and these tangles are bundles of paired helical filaments composed of hyperphosphorylated tau. In particular, phosphorylation of Ser396 by GSK-3 or CDK5 destabilizes microtubules in Alzheimer's disease. Furthermore, inclusions of tau are found in a number of other neurodegenerative diseases, collectively known as tauopathies (1, 3).1) Johnson, G. V. and Stoothoff, W. H. (2004) J. Cell Sci. 117, 5721-5729. Hanger, D. P. et al. (1998) J. Neurochem. 71, 2465-2476. Bramblett, G. T. et al. (1993) Neuron 10, 1089-1099. |
| Immunogen | Tau (phospho-Ser416) antibody was raised against a peptide sequence around phosphorylation site of serine 416 (T-G-S (p) -I-D) derived from Rat Tau. |
| Modification | p-Ser416 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Mapt |
|---|---|
| Alternative Names | pTau Mtapt RNPTAU Mapt |
| Molecular Weight(MW) | 48, 62, 78 kDa |
| Tissue Specificity | This antibody detects endogenous levels of Tau only when phosphorylated at serine 416. |
| Entrez Gene | 29477 |
|---|---|
| Protein Accession | NP_058908.2 |
Application
Western blot analysis of extract from rat brain, rat hippocampus and mouse brain using Tau (phospho-Ser416).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
