Application
Western blot analysis of TAU in SK-N-SH cell lysate with TAU antibody at 1 μg/ml.Application
Western blot analysis of TAU in (A) human and (B) rat brain tissue lysate with TAU antibody at 1 μg/mL.Application
Immunohistochemistry of TAU in mouse brain tissue with TAU antibody at 5 μg/ml.| Product Name | TAU Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Microtubial-associated protein tau, MAPT, MAPTL, DDPAC, FTDP-17L, MSTD, MTBT1, MTBT2, PPND, PPP1R103 |
| Product description | The microtubial-associated protein TAU (MAPT), more commonly known as TAU, is is normally a highly soluble protein found predominantly in neurons (1), but accumulations of highly phosphorylated tau protein aggregates are observed in several neurodegenerative diseases including Alzheimer’s disease, progressive supranuclear palsy, corticobasal degeneration, and frontotemporal lobar dementia. It was thought that these pathological tau aggregates were the toxic form of tau, but recent studies indicate that soluble and highly phosphorylated tau species are more closely associated with synaptic dysfunction and cell loss (2,3). Mutations in the TAU gene have also been associated with several of these neurodegenerative diseases (4).1) Dotti CG, Banker GA, and Binder LI. The expression and distribution of the microtubule-associated proteins tau and microtubule-associated protein 2 in hippocampal neurons in the rat in situ and in cell culture. Neuroscience 1987; 23:121-30. |
| Immunogen | TAU antibody was raised against a 19 amino acid peptide near the amino terminus of human TAU. The immunogen is located within amino acids 210 - 260 of TAU. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| TAU antibody can be used for detection of TAU by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TAU |
|---|---|
| Alternative Names | Microtubial-associated protein tau MAPT MAPTL DDPAC FTDP-17L MSTD MTBT1 MTBT2 PPND PPP1R103 |
| Molecular Weight(MW) | Predicted: 83 kDa Observed: 90 kDa |
| Sequence Similarities | 2) Cat. No. 1463 - Rat Brain Tissue Lysate |
| Tissue Specificity | TAU antibody is human, mouse and rat reactive. Multiple isoforms of TAU are known to exist; this antibody will only detect the two longest isoforms. |
Application
Western blot analysis of TAU in SK-N-SH cell lysate with TAU antibody at 1 μg/ml.
Application
Western blot analysis of TAU in (A) human and (B) rat brain tissue lysate with TAU antibody at 1 μg/mL.
Application
Immunohistochemistry of TAU in mouse brain tissue with TAU antibody at 5 μg/ml.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | TAU antibody can be used for detection of TAU by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | TAU antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | TAU antibody is supplied in PBS containing 0.02% sodium azide. |
