Application
Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | TBB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tubulin beta-1 chain, TUBB1 |
| Product description | The tubulin family of globular proteins has several members, the most common of which are a-tubulin and ?tubulin; proteins which make up microtubules of the cytoskeltons of probably all eukaryotic cells. Except in the simplest eukaryotes, tubulin (100 kDa) exists in all cells as a heterodimer of two similar but non-identical polypeptides (55 kDa each), designated alpha and beta. Within either family of alpha/beta tubulin heterodimers, individual subunits diverge from each other (both within and across species) at less than 10% of the amino acid positions. The most extreme diversity is localized to the carboxyl-terminal 15 residues. Delta (d) and epsilon (e) tubulin have been found to localize at centrioles and may play a role in forming the mitotic spindle during mitosis, though neither is as well-studied as the a- and ? forms.1) Rogowski K., et.al., Cell 137:1076-1087(2009). |
| Immunogen | This TBB1 antibody is generated from rabbits immunized with human TBB1 recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TUBB1 |
|---|---|
| Alternative Names | Tubulin beta-1 chain TUBB1 |
| Molecular Weight(MW) | 50 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: CCRF-CEM whole cell lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometry analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
